International Journal of Preventive Medicine (Int J Prev Med)2008-7802002015041114991499EN20150411<strong>Objective:</strong> To examine vertical transmission rates of Cytomegalovirus, Toxoplasma Gondii and Rubella infections according to amniotic fluid PCR analysis.<br /><strong>Methods:</strong> A retrospective analysis of mid-trimester amniocenteses performed in in pregnancies with diagnosed maternal infection by Cytomegavirus (CMV), Rubella or Toxoplasma gondii during 1994-2008 was performed. Vertical transmission rates were observed according to the presence of<br />the infectious agent&rsquo;s DNA in the amniotic fluid. A univariate regression model was also performed to investigate possible correlations between transmission and epidemiological parameters.<br /><strong>Results:</strong> Overall, 7033 amniocenteses were performed during study&rsquo;s period, of which 166 (2.4%) with the indication of maternal infection by CMV, Rubella or Toxoplasma. Mean maternal age was 27.4 &plusmn; 2.5 years and the mean gestational age at amniocentesis was 18.7 &plusmn; 2.5 weeks. Vertical<br />transmission was observed in 21 cases (12.7%). Transmission rate was 17.3% in cases with infection from CMV, 9.5% from Toxoplasma gondii and 7.8% from Rubella (P = .05). Maternal age was the only parameter being significantly associated with increased risk for vertical transmission (P = .04).<br /><strong>Conclusions:</strong> According to our results, overall vertical transmission rate marginally exceeds 10%. CMV infection is characterized by relatively higher transplacental transmission rate, while increased maternal age appears to be associated with a higher risk for vertical transmission<br /><strong>Keywords:</strong> Amniocentesis, cytomegalovirus, infection, rubella, toxoplasma, vertical transmissionhttp://ijpm.mui.ac.ir/index.php/ijpm/article/view/1499http://ijpm.mui.ac.ir/index.php/ijpm/article/download/1499/1781