International Journal of Preventive Medicine (Int J Prev Med)2008-7802112015122816211621ENWater and Electrolytes Research Center, Isfahan University of Medical Sciences, IsfahanWater and Electrolytes Research Center, Isfahan University of Medical Sciences, IsfahanWater and Electrolytes Research Center, Isfahan University of Medical Sciences, IsfahanWater and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan & Department of Physiology, Faculty of Medicine, Isfahan University of Medical Sciences, IsfahanDepartment of Clinical Pathology, Faculty of Medicine, Isfahan University of Medical Sciences, IsfahanWater and Electrolytes Research Center, Isfahan University of Medical Sciences, IsfahanWater and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan20151228<p><strong>Background:</strong> Renal ischemia/reperfusion injury (RIRI) is the most common cause of acute kidney injury. We tested the protective role of endothelin‑1 receptor blocker; bosentan (BOS) in animal model of RIRI in two different genders.</p><p><strong>Methods:</strong> Male and female Wistar rats were assigned as sham operated (sham), control group (ischemia), and case group (ischemia + BOS) treated with BOS (50 mg/kg) 2 h before bilateral kidney ischemia induced by clamping renal vessels for 45 min followed by 24 h of renal reperfusion.</p><p><strong>Results:</strong> The RIRI significantly increased the serum levels of blood urea nitrogen and creatinine in both genders (P &lt; 0.05). These values were significantly decreased by BOS in both genders. In male rats, the serum levels of malondialdehyde in the ischemia + BOS group were decreased significantly when compared with ischemia group (P &lt; 0.05).</p><p><strong>Conclusions:</strong> BOS can be used in both genders to attenuate kidney ischemia injury possibly<br />due to its effect in the renal vascular system.</p><p><strong>Keywords:</strong> Bosentan, endothelin, gender, ischemia‑reperfusion, kidney</p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/1621http://ijpm.mui.ac.ir/index.php/ijpm/article/download/1621/1912