International Journal of Preventive Medicine (Int J Prev Med)2008-7802112017061017991799ENResearch Center for Biochemistry and Nutrition in Metabolic DiseasesResearch Center for Biochemistry and Nutrition in Metabolic DiseasesResearch Center for Biochemistry and Nutrition in Metabolic DiseasesDepartment of Research and Development of Sekkeh Gaz Company, IsfahanDepartment of Infectious Disease, School of Medicine, Kashan University of Medical Sciences, KashanDepartment of Cardiology, School of Medicine, Kashan University of Medical Sciences, KashanObesity and Eating Habits Research Center, Endocrinology and Metabolism Molecular‑Cellular Sciences Institute AND Department of Community Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran AND Department of Community Nutrition, School of Nutrition and Food Science, Isfahan University of Medical Sciences, Isfahan20170610<p><span class="fontstyle0"><strong>Background</strong>: </span><span class="fontstyle2">This research was to examine the effects of synbiotic intake on minerals, liver enzymes, and blood pressure in patients with type 2 diabetes (T2D).</span></p><p><span class="fontstyle2"> </span><span class="fontstyle0"><strong>Methods</strong>: </span><span class="fontstyle2">This randomized, cross‑over clinical trial was performed among 62 diabetic patients. Persons were randomly assigned to intake either a synbiotic (</span><span class="fontstyle3">n </span><span class="fontstyle2">= 62) or a control food (</span><span class="fontstyle3">n </span><span class="fontstyle2">= 62) for 6 weeks. A 3‑week washout<br />period was applied following which persons were crossed over to the alternate intervention arm for an additional 6 weeks. The synbiotic was consisted of </span><span class="fontstyle3">Lactobacillus sporogenes </span><span class="fontstyle2">(1 &times; 10</span><span class="fontstyle2" style="font-size: 5pt;">7 </span><span class="fontstyle2">CFU), 0.04 g inulin (HPX) as prebiotic. Persons were asked to consume the synbiotic and control foods 27 g a day. Blood pressure was measured, and blood samples were taken at baseline and after 6‑week intervention to assess calcium, magnesium, iron, alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase, and total bilirubin. </span></p><p><strong><span class="fontstyle0">Results: </span></strong><span class="fontstyle2">The consumption of a synbiotic food, compared to the control food, resulted in a signifcant rise of calcium (0.66 vs. -0.14 mg/dL, </span><span class="fontstyle3">P </span><span class="fontstyle2">= 0.03) and iron (5.06 vs. -9.98 mg/dL, </span><span class="fontstyle3">P </span><span class="fontstyle2">= 0.03). The decrease of total<br />bilirubin (0.08 vs. -0.04 mg/dL; </span><span class="fontstyle3">P </span><span class="fontstyle2">= 0.009) was also seen in the synbiotic group compared with the control group. </span></p><p><span class="fontstyle0"><strong>Conclusions</strong>: </span><span class="fontstyle2">Overall, synbiotic in T2D patients had benefcial effects on calcium, iron, and total bilirubin concentrations.<br /></span></p><p><strong><span class="fontstyle0" style="color: #00652e;">Keywords: </span></strong><span class="fontstyle3">Blood pressure, liver enzymes, serum minerals, synbiotic, type 2 diabetes</span> <br style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px;" /></p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/1799http://ijpm.mui.ac.ir/index.php/ijpm/article/download/1799/2086