International Journal of Preventive Medicine (Int J Prev Med)2008-7802922018021318801880ENDepartment of Biology, Faculty of Sciences, University of Isfahan, IsfahanDepartment of Obstetrics and Gynecology, Isfahan University of Medical Sciences, Isfahanepartment of Anatomy, Faculty of Medicine, Kurdistan University of Medical Sciences, SanandajDepartment of Reproductive Biology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz20180213<p><span class="fontstyle0"><strong>Background</strong>: </span><span class="fontstyle2">Maternal thrombophilia has been identifed as a risk factor for recurrent pregnancy loss (RPL). The aim of this study was to investigate the association between prothrombin G20210A and factor V Leiden (FVL) polymorphisms in women with RPL and a control group of parous women<br />in Isfahan province of Iran.</span></p><p><strong></strong><span class="fontstyle0"><strong>Methods</strong>: </span><span class="fontstyle2">We studied 250 women with idiopathic RPL and 116 control<br />cases. Prothrombin and FVL different genotypes were determined using polymerase chain reaction and reverse hybridization technique.</span></p><p><strong></strong><span class="fontstyle0"><strong>Results</strong>: </span><span class="fontstyle2">The frequencies of heterozygous mutation prothrombin G20210A were 6% and 0.9%, respectively (</span><span class="fontstyle3">P </span><span class="fontstyle2">= 0.025), in cases compared to the control group. The frequencies of homozygous mutation prothrombin G20210A were 0.4% and 0%, respectively, in cases compared to controls (</span><span class="fontstyle3">P </span><span class="fontstyle2">= 0.02). The prothrombin mutation was signifcantly higher in cases compared to the control group (odds ratio 8.81; 95% confdence interval: 1.16–66.62). There was no signifcant difference between the FVL mutation and pregnancy loss. </span></p><p><span class="fontstyle0"><strong>Conclusions</strong>: </span><span class="fontstyle2">The results indicated a signifcant higher frequency of prothrombin G20210A in women with RPL in comparison with controls. Our data suggest that the prothrombin G20210A mutation, but not the FVL mutation, may be an unrecognized cause of RPL in our population. </span></p><p><strong></strong><span class="fontstyle0" style="color: #00652e;"><strong>Keywords</strong>: </span><span class="fontstyle3">Abortion, factor V, mutation, thrombophilia, pregnancy, prothrombin</span> </p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/1880http://ijpm.mui.ac.ir/index.php/ijpm/article/download/1880/2163