International Journal of Preventive Medicine (Int J Prev Med)2008-7802932018031819011901ENDepartment of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, IsfahanDepartment of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, IsfahanDepartment of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan20180318<p><span class="fontstyle0"><strong>Background</strong>: </span><span class="fontstyle2">This study was aimed to screen the antihyperlipidemic effect of different fractions of </span><span class="fontstyle3">Teucrium polium </span><span class="fontstyle2">to obtain the most effcient herbal fraction for isolation of bioactive constituents responsible for hypolipidemic activity. </span></p><p><strong></strong><span class="fontstyle0"><strong>Methods</strong>: </span><span class="fontstyle2">Chloroform, butanol, and aqueous fractions were obtained from hydroalcoholic extract of </span><span class="fontstyle3">T. polium </span><span class="fontstyle2">aerial parts using partitioning process. To induce hyperlipidemia, dexamethasone (Dex) was injected 10 mg/kg/day (s.c.) for 8 days. In the test groups, animals received 50, 100 and 150 mg/kg of </span><span class="fontstyle3">T. polium </span><span class="fontstyle2">hydroalcoholic extract and different fractions orally simultaneously with Dex. Serum lipid profle and hepatic marker enzymes were evaluated using biochemical kits. </span></p><p><span class="fontstyle0"><strong>Results</strong>: </span><span class="fontstyle2">All treatments, especially chloroform and aqueous fractions, reversed serum lipid markers in hyperlipidemic rats. Maximum reduction in triglyceride (60.2%, </span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.001) and maximum elevation in high‑density lipoprotein (HDL) (35.0%, </span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.01) was observed for<br />chloroform fraction. Maximum cholesterol‑lowering effect (29.0%, </span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.001) and maximum reduction in low‑density lipoprotein were found for hydroalcoholic extract (72.9%, </span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.001). Aqueous fraction improved all lipid markers at the highest dose. Butanol fraction decreased triglyceride at the lowest dose (43.9%, </span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.001) and increased HDL (33%, </span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.05) at the highest dose. There was a signifcant increase in alanine aminotransferase and aspartate aminotransferase levels in all tested groups compared to normal group (</span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.001). </span></p><p><span class="fontstyle0"><strong>Conclusions</strong>: </span><span class="fontstyle2">This study showed strong antihyperlipidemic effect of various fractions derived from hydroalcoholic extract of </span><span class="fontstyle3">T. polium</span><span class="fontstyle2">.<br />Chloroform and aqueous fractions may be worthy candidates for isolation of bioactive hypolipidemic constituents. However, possible hepatotoxicity should be considered for clinical application.<br /></span></p><p><span class="fontstyle0" style="color: #00652e;"><strong>Keywords</strong>: </span><span class="fontstyle3">Dexamethasone, hepatotoxicity, hyperlipidemia, Teucrium polium</span> </p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/1901http://ijpm.mui.ac.ir/index.php/ijpm/article/download/1901/717717725