International Journal of Preventive Medicine (Int J Prev Med)2008-7802942018041619081908ENDental Research Center, School of Dentistry, Isfahan University of Medical Sciences, IsfahanDental Materials Research Center, School of Dentistry, Isfahan University of Medical Sciences, IsfahanBiotechnology Research Center, Pasteur Institute of Iran, TehranDepartment of Prosthodontics, Student’s Research Committee, School of Dentistry, Isfahan University of Medical Sciences, IsfahanDental Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan20180416<p><span class="fontstyle0"><strong>Background</strong>: </span><span class="fontstyle2">Disinfection of dental impression is mandatory for preventing the cross infection between dental staff and dental laboratory. Ozone as a disinfectant can be used in various ways in dentistry. The aim of this study was to evaluate the efficacy of ozonated water for disinfection of irreversible hydrocolloid impression materials and preventing cross-contamination.</span></p><p><strong></strong><span class="fontstyle0"><strong>Methods</strong>: </span><span class="fontstyle2">Thirty-two circular specimens (1 cm diameter and 2 mm thickness) of irreversible hydrocolloid impression material were made. Thirty specimens were contaminated with </span><span class="fontstyle3">Pseudomonas aeruginosa </span><span class="fontstyle2">(ATCC<br />27853), </span><span class="fontstyle3">Staphylococcus aureus </span><span class="fontstyle2">(ATCC 51299), and </span><span class="fontstyle3">Candida albicans </span><span class="fontstyle2">(PTCC 5027). Except control samples (</span><span class="fontstyle3">n </span><span class="fontstyle2">= 2), the other specimens were immersed in ozonated water for 5 and 10 min (5 sample each group). Trypsin was used for separating microorganisms. Diluted microbial solution was analyzed microbiologically for colony-forming units (CFUs). Two-way repeated measure ANOVA and Scheffe post hoc test were used for data analysis (α = 0.05). </span></p><p><strong></strong><span class="fontstyle0"><strong>Results</strong>: </span><span class="fontstyle2">The number of CFUs after disinfection with ozonated water was decreased significantly (11.84%, 61.55%, and 20.27% after 5 min and 11.03%, 14.50%, and 16.99% after 10 min for </span><span class="fontstyle3">P. aeruginosa, S. aureus</span><span class="fontstyle2">, and </span><span class="fontstyle3">C. albicans</span><span class="fontstyle2">, respectively) (</span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.001). Increasing the time of disinfection resulted in significant reduction in a number of </span><span class="fontstyle3">S. aureus </span><span class="fontstyle2">only (</span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.001) although they did not vanish microorganisms completely.<br /></span></p><p><span class="fontstyle0"><strong>Conclusions</strong>: </span><span class="fontstyle2">Immersion of irreversible hydrocolloid impression material in ozonated water for 10 min will not lead to complete disinfection but decreases the microorganisms to a level that can prevent infection transmission.<br /></span></p><p><span class="fontstyle0" style="color: #00652e;"><strong>Keywords</strong>: </span><span class="fontstyle3">Colony count, dental impression materials, disinfection, infection control</span> </p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/1908http://ijpm.mui.ac.ir/index.php/ijpm/article/download/1908/717717733