International Journal of Preventive Medicine (Int J Prev Med)2008-78021022019021920087802ENDepartment of Biology, Faculty of Basic Sciences, Bu‑Ali Sina University, HamedanDepartment of Biology, Faculty of Basic Sciences, Bu‑Ali Sina University, HamedanDepartment of Biology, Faculty of Basic Sciences, Bu‑Ali Sina University, HamedanMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, ShahrekordDepartment of Internal Medicine, Isfahan University of Medical Sciences, Isfahan20190218<p><span class="fontstyle0"><strong>Background</strong>: </span><span class="fontstyle2">The aim of this study was to investigate the effect of hydroethanolic </span><span class="fontstyle3">Nigella sativa </span><span class="fontstyle2">L. extract on skin wound healing in diabetic male rats. </span></p><p><span class="fontstyle0">Methods</span><span class="fontstyle2">: This experimental study was conducted on 49 male Wistar rats weighing 220–250 g divided into 7 groups of 7 each: control (nondiabetic<br />untreated), sham (nondiabetic eucerin‑treated), nondiabetic phenytoin (1%)‑treated, diabetic untreated, and three diabetic groups treated independently with phenytoin 1%, hydroethanolic </span><span class="fontstyle3">N</span><span class="fontstyle2">. </span><span class="fontstyle3">sativa </span><span class="fontstyle2">extracts 20% or 40%. Diabetes was induced with 60 mg/kg streptozosin in one administration. After anesthesia, 2 × 1 cm</span><span class="fontstyle2" style="font-size: 5pt;">2 </span><span class="fontstyle2">wounds were made on the rats’ backs and each group was administered with its own respective treatment until the wounds were healed completely. Tissue specimens were prepared<br />for histological examinations. The areas of the wounds were measured every 3 days. The data were analyzed by ANOVA and Tukey’s post‑hoc test. </span></p><p><span class="fontstyle0"><strong>Results</strong>: </span><span class="fontstyle2">The mean duration of wound healing was 27 and 24 days for diabetic untreated and diabetic phenytoin‑treated groups, respectively. Wounds were healed completely in nondiabetic untreated, sham, and nondiabetic phenytoin‑treated groups on days 23, 24, and 21, respectively. The shortest duration of wound healing was seen in diabetic </span><span class="fontstyle3">N</span><span class="fontstyle2">. </span><span class="fontstyle3">sativa<br /></span><span class="fontstyle2">extract (40%)‑treated group (15 days) followed by diabetic </span><span class="fontstyle3">N</span><span class="fontstyle2">. </span><span class="fontstyle3">sativa </span><span class="fontstyle2">(20%)‑treated group (18 days). These two groups were found to have the lowest mean wound area during the study with a signifcant difference from mean wound area in the controls (</span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.05). </span></p><p><strong></strong><span class="fontstyle0"><strong>Conclusions</strong>: </span><span class="fontstyle3">N</span><span class="fontstyle2">. </span><span class="fontstyle3">sativa </span><span class="fontstyle2">extract signifcantly promoted wound healing in diabetic rats in comparison with control groups. Although the benefcial mechanism of the promotion of wound healing was not specifcally studied, it is believed that the anti‑inflammatory and antimicrobial properties of </span><span class="fontstyle3">N</span><span class="fontstyle2">. </span><span class="fontstyle3">sativa </span><span class="fontstyle2">would contribute to this enhanced wound healing.<br /></span></p><p><span class="fontstyle0" style="color: #00652e;"><strong>Keywords</strong>: </span><span class="fontstyle3">Diabetes, Nigella sativa L., rat, skin wound</span></p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/2005http://ijpm.mui.ac.ir/index.php/ijpm/article/download/2005/717717826