International Journal of Preventive Medicine (Int J Prev Med)2008-780210102020020921672167ENRazi Herbal Researches Center & Department of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, KhorramabadDepartment of Biochemistry, Faculty of Medicine, Lorestan University of Medical Sciences, Khorramabad & Department of Biochemistry, Faculty of Medicine, Ardabil; University of Medical Sciences, Ardabil20200126<p><span class="fontstyle0"><strong>Background</strong>: </span><span class="fontstyle2">We investigated the effect of thioctic acid (TA) on kidney function, oxidative stress, and inflammatory status in serum and kidney homogenates of a rat subjected to ischemia–reperfusion injury (IRI). </span></p><p><span class="fontstyle0"><strong>Materials and Methods:</strong> </span><span class="fontstyle2">Thirty male Wistar rats were randomly divided into three<br />equal groups: sham, IR, and IR + TA in 50 mg/kg once‑daily intraperitoneal injection for 2 weeks, before IR induction. The levels of urea and creatinine (Cr) in the serum of rats were measured. Malondialdehyde and nitric oxide (NO) as stress oxidative markers; tumor necrosis factor‑</span><span class="fontstyle3">α</span><span class="fontstyle2">, interleukin‑6, and myeloperoxidase as inflammatory markers, as well as activities of superoxide dismutase, glutathione peroxidase and catalase, and glutathione (GSH) level in both serum and kidney homogenates were determined. </span></p><p><span class="fontstyle0"><strong>Results</strong>: </span><span class="fontstyle2">Cr and urea increased in serum of IR group. Furthermore, levels of oxidative stress and inflammatory markers in serum and kidney homogenates of the cited group were higher than the sham group. TA not only decreased the levels of Cr, urea, oxidative stress, and inflammation but also elevated the level of GSH and activities of antioxidant enzymes (</span><span class="fontstyle4">P </span><span class="fontstyle2">&lt; 0.001). </span></p><p><span class="fontstyle0"><strong>Conclusions</strong>: </span><span class="fontstyle2">The findings showed that TA protected IR rat against kidney dysfunction and IRI due to reinforcing endogenous antioxidant and subtracting of inflammatory markers.<br /></span></p><p><span class="fontstyle0" style="color: #00652e;"><strong>Keywords</strong>: </span><span class="fontstyle4">Antioxidant enzymes, inflammation, renal ischemia–reperfusion, oxidative stress, thioctic acid</span></p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/2167http://ijpm.mui.ac.ir/index.php/ijpm/article/download/2167/717717963