International Journal of Preventive Medicine (Int J Prev Med)2008-78021122020042022332233ENDepartment of Biology, Damghan Branch, Islamic Azad University, DamghanMedical Biology Research Center, Kermanshah University of Medical Sciences, KermanshahDepartment of Biology, Damghan Branch, Islamic Azad University, DamghanMedical Biology Research Center, Kermanshah University of Medical Sciences, KermanshahDepartment of Biology, Damghan Branch, Islamic Azad University, Damghan20200420<p><span class="fontstyle0"><strong>Background</strong>: </span><span class="fontstyle2">Renal ischemia‑reperfusion disturbs both the function and the histology of this<br />organ. Acacetin (Aca) is a natural flavonoid that is effective for relief of many diseases. The<br />aim of this study was to determine the impacts of Aca on renal ischemia‑reperfusion process in<br />mice. </span></p><p><span class="fontstyle0"><strong>Methods</strong>: </span><span class="fontstyle2">In total, 84 male Balb/cmice divided into 12 groups and were administrated<br />intraperitoneally for 4 days with or without surgery to dimethyl sulfoxide 0.01% or Aca (10, 25,<br />and 50 mg/kg) as Control, control Acas, sham, sham Acas groups. Ischemia‑reperfusion without<br />or with Aca (10, 25, and 50 mg/kg) treatments were the other groups. Parameters related to the<br />function and the histology of the kidneys were evaluated and statistically analyzed from kidney<br />and blood serum samples in the respect of the groups. </span></p><p><span class="fontstyle0"><strong>Results</strong>: </span><span class="fontstyle2">In ischemia‑reperfusion and<br />ischemia‑reperfusion + Aca (10 mg/kg) groups, there were significantly increased in urea, creatinine,<br />malondialdehyde (MDA), and apoptosis rate, whereas total antioxidant capacity decreased compared<br />to the control and sham and ischemia‑reperfusion + Aca (25 and 50 mg/kg) (</span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.05). The<br />histopathology alteration was seen in the ischemia‑reperfusion group than the others (</span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.01).<br />Moreover, there was a significant difference between ischemia‑reperfusion + Aca (25 and50 mg/kg)<br />groups than ischemia‑reperfusion + Aca (10 mg/kg) one (</span><span class="fontstyle3">P </span><span class="fontstyle2">&lt; 0.05). </span></p><p><span class="fontstyle0"><strong>Conclusions</strong>: </span><span class="fontstyle2">The recovery<br />effect of Aca was offered on renal ischemia‑reperfusion damage in a dose‑dependent manner in mice,<br />showing by kidney histopathology and functional criteria improvements. The attributed mechanism<br />for this impression would be the antioxidant property of Aca, decreasing both MDA levels and<br />apoptosis rate in kidney tissue.<br /></span></p><p><span class="fontstyle0" style="color: #00652e;"><strong>Keywords</strong>: </span><span class="fontstyle3">Acacetin, antioxidants, apoptosis, malondialdehyde, reperfusion injury</span></p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/2233http://ijpm.mui.ac.ir/index.php/ijpm/article/download/2233/717718053