International Journal of Preventive Medicine (Int J Prev Med)2008-78021182020092723262326ENDepartment of Physical Education, Sharif University of Technology TehranDepartment of Exercise Physiology, Faculty of Physical Education and Sport Sciences. University of Tehran, TehranDepartment of Exercise Physiology, Faculty of Physical Education and Sport Sciences. University of Tehran, TehranDepartment of Physical Education, Sharif University of Technology Tehran20200927<p><strong>Background</strong>: Pathological hypertrophy is one of the negative consequences of cardiac sympathetic hyperactivity. Recent studies have shown that YAP1 plays a critical role in cardiomyocytes<br />hypertrophy. Considering the preventive role of exercise training in cardiovascular diseases, the<br />present study was conducted to examine the effect of aerobic exercise training on YAP1 gene<br />expression and its upstream components.</p><p><strong> Methods</strong>: Eighteen male Wistar rats were randomly divided<br />into aerobic training and control groups. Aerobic training was performed one hour/day, five days per<br />week, for eight weeks, on a treadmill at 65‑75% VO2 max. Pathological hypertrophy was induced<br />by injecting 3 mg/kg‑1 of isoproterenol for seven days. The left ventricle was separated, and YAP1,<br />3‑mercaptopyruvate sulfurtransferase (MST), large tumor suppressor (LATS), and mitogen‑activated<br />protein 4 kinase (MAP4K) gene expressions were assessed and YAP1 protein levels were also<br />assessed by western blotting. Cell apoptosis was detected by TUNEL assays. The between‑group<br />differences were evaluated using the T‑test and P value &lt;0.05 was considered statistically significant.</p><p><strong>Results</strong>: There were no significant between‑group differences in MST gene expression (P = 0.061);<br />meanwhile, in the training group, LATS and Map4K expressions were suppressed, followed by<br />a significant increase in YAP1 expression (P &lt; 0.001). Compared to the control group, the left<br />ventricular weight increased significantly in the training group while the cardiomyocyte apoptosis<br />decreased.</p><p><strong>Conclusions</strong>: The results showed that, by reducing LATS, aerobic training‑induced<br />YAP1 upregulation can help prevent the propagation of cardiomyocyte apoptosis due to pathological<br />conditions.<br /><br /></p>http://ijpm.mui.ac.ir/index.php/ijpm/article/view/2326http://ijpm.mui.ac.ir/index.php/ijpm/article/download/2326/717718168