International Journal of Preventive Medicine (Int J Prev Med)2008-78021182020092723292329ENPharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad & Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad,Department of Pharmacology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad,Department of Medical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, MashhadNanotechnology Research Center, Institute of Pharmaceutical Technology, Mashhad University of Medical Sciences, MashhadPharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad20200927Introduction: Cardiovascular disorders (CVD) are a common cause of mortality worldwide. Oxidative<br />stress is thought to be a major factor leading to CVD. Anti‑oxidants such as medicinal plants may<br />have a role in the mitigation of vascular problems through free radicals scavenging. In this study, we<br />evaluated the protective effects of Rheum turkestanicum against hydrogen peroxide (H2O2)‑induced<br />toxicity in endothelial cells (BAE‑1). Methods: To evaluate the protective effect of R. turkestanicum<br />against H2O2 toxicity, four groups comprised of control group (the cells without any treatment),<br />H2O2 group (the cells incubated with H2O2 (200 μM)), and treatment groups (the cells treated with<br />R. turkestanicum (12200 μg/ml) alone or 24h before exposure to H2O2). Quercetin (30.23 μg/ml)<br />was used as a bioactive ingredient of the extract. Then the cell viability, reactive oxygen species,<br />lipid peroxidation, and apoptosis were evaluated. Results: H 2O2 exposure reduced cell viability to<br />13.6 ± 1.6%, enhanced ROS generation to 1445 ± 80.7%, lipid peroxidation (LPO, 290 ± 13% of<br />control), and apoptotic cells (P &lt; 0.001). In contrast, compared with H2O2 group, R. turkestanicum<br />and quercetin significantly restored the cell viability to 80.3 ± 1.6 and 87.2 ± 2.1%, ROS<br />formation to 186 ± 10 and 129 ± 1%, as well as LPO to 130.7 ± 7.7 and 116 ± 2.5 of control,<br />respectively (P &lt; 0.001). Therefore, the extract reduced H2O2‑induced toxicity in BAE‑1 cells by<br />scavenging of free radicals. Conclusion: Our findings demonstrated that the extract might reduce<br />toxicity of endothelial cells by attenuation of oxidative stress, which can be related to the presence<br />of active ingredients including quercetin.<br /><br />http://ijpm.mui.ac.ir/index.php/ijpm/article/view/2329http://ijpm.mui.ac.ir/index.php/ijpm/article/download/2329/717718171