International Journal of Preventive Medicine (Int J Prev Med)2008-780212122022011725752575ENFaculty of Medicine, Jordan University of Science and TechnologyFaculty of Applied Medical Sciences, Jordan University of Science and TechnologyFaculty of Medicine, Yarmouk University, IrbidFaculty of Applied Medical Sciences, Jordan University of Science and TechnologyFaculty of Medicine, Jordan University of Science and TechnologyFaculty of Medicine, Jordan University of Science and TechnologyFaculty of Medicine, Jordan University of Science and Technology20220117Background: Congenital hearing loss is one of the important illnesses that affect newborns. Early diagnosis and treatment are a challenge for medical authorities in developing countries to improve children’s functional, intellectual, emotional, and social abilities. We aimed to study the prevalence of congenital hearing loss in northern Jordan community and identify factors that could affect hearing screening protocol. Methods: Prospective cross‑sectional study of 1595 infants born in our hospital underwent hearing screening tests. Totally, 104 were tested in NICU and the rest examined in the nursery room using Otoacoustic emission (OAE) test as a primary testing tool. The patients were followed in the three hearing screening phases. Factors affecting screening results were studied and analyzed. Results: The total number of newborns who didn’t pass the first OAE test in one or both ears were 90 (5.6%); 69 from the nursery group and 21 from the NICU group. In the 2nd screening phase 21 (23.3%) didn’t attend the appointment. Sixty‑four passed the second screening OAE test. Five newborns (5.6%) had a second refer result in one or both ears and referred for a diagnostic ABR test. Three infants passed the test and two found to have bilateral hearing loss. Conclusions: Hearing screening test is conducted via a 3‑phases‑protocol. OAE is used in the first two phases and ABR in the third phase. Hearing results is significantly affected for infants admitted to NICU. The following factors increase OAE fail response: mechanical ventilation for more than 5 days, Hyperbilirubinemia, associated congenital anomalies. Mode of delivery doesn’t have statistical significance on hearing screening results.http://ijpm.mui.ac.ir/index.php/ijpm/article/view/2575http://ijpm.mui.ac.ir/index.php/ijpm/article/download/2575/717718415