International Journal of Preventive Medicine (Int J Prev Med)2008-78021322022040926132613ENDepartment of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, IsfahanDepartment of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan,Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan20220406<strong>Background</strong>: Oxidative stress has a prominent role in the pathogenesis of diabetes complications. Pramlintide is an injectional amylin analogue used for the treatment of type 1 and type 2 diabetic patients. The present investigation evaluated the effect of pramlintide against oxidative damage induced by hydrogen peroxide (H2 O2 ) in human umbilical vein endothelial cells (HUVECs). <strong>Methods</strong>: Cell viability was assessed using 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide method. Hydroperoxides level, ferric reducing antioxidant power (FRAP), and expression of transcription factor NF‑κB were measured in HUVECs that pretreated with pramlintide and, then exposed to H2 O2 . <strong>Results</strong>: Pramlintide significantly decreased the cytotoxicity caused by H2 O2 at the concentrations of 5 and 10 µg/mL. Pretreatment of HUVECs with pramlintide reduced hydroperoxides and increased FRAP value in intra‑ and extra‑cellular mediums at different concentration ranges compared with H2 O2 stimulated cells. Pramlintide (10 µg/mL) remarkably ameliorated the expression of NF‑κB gene after 1, 3 and 24 h exposure to H2 O2 . <strong>Conclusions</strong>: Findings of the current investigation displayed that pramlintide may act as a protective against oxidative conditions in endothelial cells through modulation of oxidative markers and transcription factor NF‑κB.http://ijpm.mui.ac.ir/index.php/ijpm/article/view/2613http://ijpm.mui.ac.ir/index.php/ijpm/article/download/2613/717718453