International Journal of Preventive Medicine (Int J Prev Med)2008-78024520120915517523ENNeurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, IranPhysiology Reseach Center, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Pharmacology, School of Medicine, Guilan University of Medical Sciences, Rasht, IranDepartment of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center and, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, IranPhysiology Reseach Center, Isfahan University of Medical Sciences, Isfahan, IranSedigheh Tahereh Research Complex, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran20120915<p><strong>Background: </strong>It is believed that paraoxonase‑2 gene polymorphism is associated with type 2 diabetes. This study is aimed to investigate the association between paraoxonase‑2 gene polymorphism and type 2 diabetes in an Iranian population.</p> <p><strong>Methods: </strong>This study was performed on 200 individuals including 100 diabetics and 100 healthy subjects. Genomic DNA was extracted from peripheral blood leukocytes, and PCR‑RFLP was carried out. Palindromic sequence in PON2 gene was recognized by Dde1 restriction endonuclease. In order to visualize restriction products, electrophoresis was carried out using polyacrylamide gel (8%) and ethidium bromide staining.</p> <p><strong>Results: </strong>The expected PCR product of 331 bp was obtained. Digestion of this product with DdeI showed four Ser homozygotes, three Cys homozygotes, and five Ser311 Cys heterozygotes. The gene frequency of Cys (C) in diabetic subjects was significantly higher than in healthy subjects.</p> <p><strong>Conclusions: </strong>This study suggests that an association exists between Ser311 Cys polymorphism and type 2 diabetes mellitus.</p> <strong>Keywords: </strong>Iranian population, paraxonase‑2 gene, polymorphism, type 2 diabeteshttp://ijpm.mui.ac.ir/index.php/ijpm/article/view/910http://ijpm.mui.ac.ir/index.php/ijpm/article/download/910/1045