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Administration of Lactobacillus casei and Bifidobacterium bifidum Ameliorated Hyperglycemia, Dyslipidemia, and Oxidative Stress in Diabetic Rats
Abstract
Background: The present work was planned to evaluate the antihyperglycemic, lipid‑lowering, and
antioxidant effect of Lactobacillus casei and Bifidobacterium bifidum in streptozotocin (STZ)‑induced
diabetic rats.
Methods: Single daily dose of 1 × 107 cfu/ml of L. casei and B. bifidum alone and in combination of
both was given to Wistar rats orally by gavaging for 28 days. Glucose tolerance test, fasting blood
glucose (FBG), lipid profile, and glycosylated hemoglobin (HbA1c) were measured from blood.
Glycogen from thigh muscles and liver and oxidative stress parameters from pancreas were analyzed.
Results: Administration of L. casei and B. bifidum alone and in combination of both to diabetic rats
decreased serum FBG (60.47%, 55.89%, and 56.49%, respectively), HbA1c (28.11%, 28.61%,
and 28.28%), total cholesterol (171.69%, 136.47%, and 173.58%), triglycerides (9.935%, 8.58%,
and 7.91%), low‑density lipoproteins (53.27%, 53.35%, and 52.91%) and very low‑density
lipoproteins (10%, 8.58%, and 11.15%, respectively) and increased high‑density lipoproteins (13.73%,
15.47%, and 15.47%), and insulin (19.50%, 25.80%, and 29.47%, respectively). The treatment also
resulted in increase in muscle (171.69%, 136.47%, and 173.58%) and liver (25.82%, 6.63%, and
4.02%) glycogen level. The antioxidant indexes in pancreas of diabetic rats returned to normal
level with reduction in lipid peroxidation (30.89%, 46.46%, and 65.36%) and elevation in reduced
glutathione (104.5%, 161.34%, and 179.04%), superoxide dismutase (38.65%, 44.32%, and 53.35%),
catalase (13.08%, 27%, and 31.52%), glutathione peroxidase (55.56%, 72.23%, and 97.23%),
glutathione reductase (49.27%, 88.40%, and 110.86%), and glutathione‑S‑transferase (140%,
220%, and 246.6%, respectively) on treatment with L. casei, B. bifidum, and combination treatment.
Conclusions: Administration of L. casei and B. bifidum alone and in combination of both
ameliorated hyperglycemia, dyslipidemia, and oxidative stress in STZ‑induced diabetic Wistar rats.
Keywords: Antihyperglycemic, antioxidant, Bifidobacterium bifidum, dyslipidemia, Lactobacillus
casei
antioxidant effect of Lactobacillus casei and Bifidobacterium bifidum in streptozotocin (STZ)‑induced
diabetic rats.
Methods: Single daily dose of 1 × 107 cfu/ml of L. casei and B. bifidum alone and in combination of
both was given to Wistar rats orally by gavaging for 28 days. Glucose tolerance test, fasting blood
glucose (FBG), lipid profile, and glycosylated hemoglobin (HbA1c) were measured from blood.
Glycogen from thigh muscles and liver and oxidative stress parameters from pancreas were analyzed.
Results: Administration of L. casei and B. bifidum alone and in combination of both to diabetic rats
decreased serum FBG (60.47%, 55.89%, and 56.49%, respectively), HbA1c (28.11%, 28.61%,
and 28.28%), total cholesterol (171.69%, 136.47%, and 173.58%), triglycerides (9.935%, 8.58%,
and 7.91%), low‑density lipoproteins (53.27%, 53.35%, and 52.91%) and very low‑density
lipoproteins (10%, 8.58%, and 11.15%, respectively) and increased high‑density lipoproteins (13.73%,
15.47%, and 15.47%), and insulin (19.50%, 25.80%, and 29.47%, respectively). The treatment also
resulted in increase in muscle (171.69%, 136.47%, and 173.58%) and liver (25.82%, 6.63%, and
4.02%) glycogen level. The antioxidant indexes in pancreas of diabetic rats returned to normal
level with reduction in lipid peroxidation (30.89%, 46.46%, and 65.36%) and elevation in reduced
glutathione (104.5%, 161.34%, and 179.04%), superoxide dismutase (38.65%, 44.32%, and 53.35%),
catalase (13.08%, 27%, and 31.52%), glutathione peroxidase (55.56%, 72.23%, and 97.23%),
glutathione reductase (49.27%, 88.40%, and 110.86%), and glutathione‑S‑transferase (140%,
220%, and 246.6%, respectively) on treatment with L. casei, B. bifidum, and combination treatment.
Conclusions: Administration of L. casei and B. bifidum alone and in combination of both
ameliorated hyperglycemia, dyslipidemia, and oxidative stress in STZ‑induced diabetic Wistar rats.
Keywords: Antihyperglycemic, antioxidant, Bifidobacterium bifidum, dyslipidemia, Lactobacillus
casei