Prevention of Cross-contamination Risk by Disinfection of Irreversible Hydrocolloid Impression Materials with Ozonated Water

Omid Savabi, Farahnaz Nejatidanesh, Kamran Poshang Bagheri, Leila Karimi, Ghazal Savabi

Abstract


Background: Disinfection of dental impression is mandatory for preventing the cross infection between dental staff and dental laboratory. Ozone as a disinfectant can be used in various ways in dentistry. The aim of this study was to evaluate the efficacy of ozonated water for disinfection of irreversible hydrocolloid impression materials and preventing cross-contamination.

Methods: Thirty-two circular specimens (1 cm diameter and 2 mm thickness) of irreversible hydrocolloid impression material were made. Thirty specimens were contaminated with Pseudomonas aeruginosa (ATCC
27853), Staphylococcus aureus (ATCC 51299), and Candida albicans (PTCC 5027). Except control samples (n = 2), the other specimens were immersed in ozonated water for 5 and 10 min (5 sample each group). Trypsin was used for separating microorganisms. Diluted microbial solution was analyzed microbiologically for colony-forming units (CFUs). Two-way repeated measure ANOVA and Scheffe post hoc test were used for data analysis (α = 0.05).

Results: The number of CFUs after disinfection with ozonated water was decreased significantly (11.84%, 61.55%, and 20.27% after 5 min and 11.03%, 14.50%, and 16.99% after 10 min for P. aeruginosa, S. aureus, and C. albicans, respectively) (P < 0.001). Increasing the time of disinfection resulted in significant reduction in a number of S. aureus only (P < 0.001) although they did not vanish microorganisms completely.

Conclusions: Immersion of irreversible hydrocolloid impression material in ozonated water for 10 min will not lead to complete disinfection but decreases the microorganisms to a level that can prevent infection transmission.

Keywords: Colony count, dental impression materials, disinfection, infection control


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References


Bergman B. Disinfection of prosthodontic impression materials:

A literature review. Int J Prosthodont 1989;2:537-42.

Cottone JA, Molinari JA. Selection for dental practice of

chemical disinfectants and sterilants for hepatitis and AIDS. Aust

Dent J 1987;32:368-74.

Egusa H, Watamoto T, Matsumoto T, Abe K, Kobayashi M,

Akashi Y, et al. Clinical evaluation of the efficacy of removing

microorganisms to disinfect patient-derived dental impressions.

Int J Prosthodont 2008;21:531-8.

Nassar U, Chow AK. Surface detail reproduction and effect

of disinfectant and long-term storage on the dimensional

stability of a novel vinyl polyether silicone impression material.

J Prosthodont 2015;24:494-8.

Nejatidanesh F, Khosravi Z, Goroohi H, Badrian H, Savabi O.

Risk of contamination of different areas of dentist’s face during

dental practices. Int J Prev Med 2013;4:611-5.

Al-Jabrah O, Al-Shumailan Y, Al-Rashdan M. Antimicrobial

effect of 4 disinfectants on alginate, polyether, and polyvinyl siloxane impression materials. Int J Prosthodont

;20:299-307.

Blair FM, Wassell RW. A survey of the methods of disinfection

of dental impressions used in dental hospitals in the

United Kingdom. Br Dent J 1996;180:369-75.

Nejatidanesh F, Bagheri KP, Shahtousi M, Talaei M, Savabi O.

Disinfection of polyether impression materials with hydrogen

peroxide solution containing silver ion. J Isfahan Dent Sch

;5:181-8.

Izadi A, Farnaz F, Soufiabadi S, Vafaee F, Kasraei S. Antibacterial

effect of sanosil 2% and 6% and sodium hypochlorite 0.5%

on impressions of irreversible hydrocolloid (Alginate) and

condensational silicone (Speedex). Avicenna J Dent Res

;5:e21107.

Dorner AR, Ferraz da Silva JM, Uemura ES, Borges AL,

Fernandes Junior VB, Yamamoto EC. Effect of disinfection of

irreversible hydrocolloid impression materials with 1% sodium

hypochlorite on surface roughness and dimensional accuracy of

dental stone casts. Eur J Gen Dent 2014;3:113.

Muzaffar D, Braden M, Parker S, Patel MP. The effect of

disinfecting solutions on the dimensional stability of dental

alginate impression materials. Dent Mater 2012;28:749-55.

McNeill MR, Coulter WA, Hussey DL. Disinfection of

irreversible hydrocolloid impressions: A comparative study. Int J

Prosthodont 1992;5:563-7.

Sofou A, Larsen T, Fiehn NE, Owall B. Contamination level of

alginate impressions arriving at a dental laboratory. Clin Oral

Investig 2002;6:161-5.

Ghahramanloo A, Sadeghian A, Sohrabi K, Bidi A.

A microbiologic investigation following the disinfection of

irreversible hydrocolloid materials using the spray method.

J Calif Dent Assoc 2009;37:471-7.

Azarpazhooh A, Limeback H. The application of ozone

in dentistry: A systematic review of literature. J Dent

;36:104-16.

Bocci V, Borrelli E, Travagli V, Zanardi I. The ozone paradox:

Ozone is a strong oxidant as well as a medical drug. Med Res

Rev 2009;29:646-82.

Bocci VA. Scientific and medical aspects of ozone therapy. State

of the art. Arch Med Res 2006;37:425-35.

Seidler V, Linetskiy I, Hubálková H, Stanková H, Smucler R,

Mazánek J. Ozone and its usage in general medicine and

dentistry. A review article. Prague Med Rep 2008;109:5-13.

Nogales CG, Ferrari PH, Kantorovich EO, Lage-Marques JL.

Ozone therapy in medicine and dentistry. J Contemp Dent Pract

;9:75-84.

Arita M, Nagayoshi M, Fukuizumi T, Okinaga T, Masumi S,

Morikawa M, et al. Microbicidal efficacy of ozonated water

against Candida albicans adhering to acrylic denture plates. Oral

Microbiol Immunol 2005;20:206-10.

Anand SK, Ebenezar AV, Anand N, Mary AV, Mony B.

A comparative analysis of antimicrobial property of wine and

ozone with calcium hydroxide and chlorhexidine. J Clin Diagn

Res 2015;9:ZC04-6.

Wilczynska-Borawska M, Leszczynska K, Nowosielski C,

Stokowska W. Ozone in dentistry: Microbiological effects of

gas action depending on the method and the time of application

using the ozonytron device. Experimental study. Ann Acad Med

Stetin 2011;57:99-103.

Lynch E. Comment on “The application of ozone in dentistry:

A systematic review of the literature”. J Dent 2009;37:406-10.

Baysan A, Lynch E. Clinical reversal of root caries using ozone:

-month results. Am J Dent 2007;20:203-8.

Estrela C, Estrela CR, Decurcio Dde A, Silva JA, Bammann LL.

Antimicrobial potential of ozone in an ultrasonic cleaning system

against Staphylococcus aureus. Braz Dent J 2006;17:134-8.

Lynch E. Evidence-based caries reversal using ozone. J Esthet

Restor Dent 2008;20:218-22.

al-Omari WM, Jones JC, Hart P. A microbiological investigation

following the disinfection of alginate and addition cured silicone

rubber impression materials. Eur J Prosthodont Restor Dent

;6:97-101.

Rueggeberg FA, Beall FE, Kelly MT, Schuster GS. Sodium

hypochlorite disinfection of irreversible hydrocolloid impression

material. J Prosthet Dent 1992;67:628-31.

Hiraguchi H, Iwasaki Y, Iwasaki E, Kikuchi H, Hirose H,

Yoneyama T. Dimensional changes in stone models simulating

full crown preparations with adjacent teeth resulting from

long-term immersion of medium-viscosity addition-type silicone

rubber impressions in disinfectant solutions. Dent Mater J

;34:48-53.

Hiraguchi H, Kaketani M, Hirose H, Yoneyama T. Effect of

immersion disinfection of alginate impressions in sodium

hypochlorite solution on the dimensional changes of stone

models. Dent Mater J 2012;31:280-6.

Nejtidanesh F, Savabi O, Asgari SN, Shafie S. Dimensional

accuracy of irreversible hydrocolloid impressions disinfected

with ozonated water. J Isfahan Dent Sch 2012;8:18-26.

Salehi R, Savabi O, Kazemi M, Kamali S, Salehi AR, Eslami G,

et al. Effects of Lactobacillus reuteri-derived biosurfactant on

the gene expression profile of essential adhesion genes (gtfB,

gtfC and ftf) of Streptococcus mutans. Adv Biomed Res

;3:169.

Savabi O, Kazemi M, Kamali S, Salehi AR, Eslami G,

Tahmourespour A, et al. Effects of biosurfactant produced

by Lactobacillus casei on gtfB, gtfC, and ftf gene expression

level in S. mutans by real-time RT-PCR. Adv Biomed Res

;3:231.