Relationship between Helicobacter pylori cagA Genotypes Infection and IL‑10 and TGFβ1 Genes’ Expression in Gastric Epithelial Cells

Abstract

Background: The correlation of Helicobacter pylori infection with gastritis, peptic ulcer, and
gastric cancer has been proven. The aim of this study was to determine the effects of cagA+ and
cagA- genotypes of H. pylori on genes expression of interleukin (IL) -10 and tumor growth
factor (TGF) β1 in gastric epithelial cells of patients with gastritis and H. pylori infection.

Methods: In all, 45 gastric biopsy samples were collected from patients with gastritis and H. pylori
infection admitted to Tohid Hospital in Sanandaj city. Status of urease and cagA genes of H. pylori
were directly determined from the biopsy samples using polymerase chain reaction (PCR) method.
Expression of IL-10 and TGF-β1 genes in gastric epithelial cells of patients with gastritis and
cagA+ and cagA- genotypes of H. pylori infection was serveyed using real-time PCR method.

Results: Overall, 25 samples had infection with H. pylori cagA+ and 20 with cagA- genotypes.
This study showed that there is a positive correlation between cagA- genotypes of H. pylori and
increasing of IL-10 gene expression in gastric epithelial cells of patients with gastritis (P = 0.001).

Conclusions: Level of gene expression of IL-10 as an anti-inflammatory cytokine in gastric epithelial
cells of patients with H. pylori infection is connected to cagA‑ genotypes.

Keywords: Cytotoxin‑associated gene A, gastric epithelial cells, Helicobacter pylori, interleukin‑10,
transforming growth factor‑beta1