Chlorella sp. Protective Effect on Acetaminophen‑Induced Liver Toxicity in ICR Mice

Jia‑Ping Wu

Abstract


Background: A Chlorella sp. (CLC) has a health supplement in health effects including an ability to treat cancer. The Chlorella sp. Ability to reduce acetaminophen-induced liver injury is still unknown. The hepatoprotective function of CLC was determined in an APAP-induced liver injury mouse model.

Methods: Male ICR mice were randomly divided into normal control, APAP,
APAP + Sm (silymarin) and APAP + CLC (0.2%, 0.5% and 1%) groups. The glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), Albumin, and BUN plasma activities were detected using blood biochemistry assay. The hepatic tissue GOT, GPT, superoxide dismutase
(SOD) and catalase (CAT) activity were also detected. Lipid peroxidation, MDA, protein expression levels were examined.

Results: The results showed that the 1% CLC supplementation group and
Silymarin (Sm) could significantly alleviate increased serum GOT, GPT and BUN, and the decreased serum Albumin. At the same time, the increased hepatic tissue GOT and GPT activities were alleviated as well as MDA. Enhanced SOD and CAT protein expression levels were increased in
APAP-induced liver injury. Lipofuscin and hepatic veins cups disappeared in the Sm and 1% CLC supplementation groups shown with H&E staining.

Conclusions: Therefore, CLC probably could develop hepatoprotective products against chemical-induced liver damage.

Keywords: Acetaminophen, catalase, Chlorella sp. crude lysate, glutamic oxaloacetic transaminase,
glutamic pyruvic transaminase, hepatoprotective function, superoxide dismutase


Keywords


Acetaminophen; catalase; Chlorella sp. crude lysate; glutamic oxaloacetic transaminase; glutamic pyruvic transaminase; hepatoprotective function; superoxide dismutase

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