Chlorella sp. Protective Effect on Acetaminophen‑Induced Liver Toxicity in ICR Mice

Jia‑Ping Wu


Background: A Chlorella sp. (CLC) has a health supplement in health effects including an ability to treat cancer. The Chlorella sp. Ability to reduce acetaminophen-induced liver injury is still unknown. The hepatoprotective function of CLC was determined in an APAP-induced liver injury mouse model.

Methods: Male ICR mice were randomly divided into normal control, APAP,
APAP + Sm (silymarin) and APAP + CLC (0.2%, 0.5% and 1%) groups. The glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), Albumin, and BUN plasma activities were detected using blood biochemistry assay. The hepatic tissue GOT, GPT, superoxide dismutase
(SOD) and catalase (CAT) activity were also detected. Lipid peroxidation, MDA, protein expression levels were examined.

Results: The results showed that the 1% CLC supplementation group and
Silymarin (Sm) could significantly alleviate increased serum GOT, GPT and BUN, and the decreased serum Albumin. At the same time, the increased hepatic tissue GOT and GPT activities were alleviated as well as MDA. Enhanced SOD and CAT protein expression levels were increased in
APAP-induced liver injury. Lipofuscin and hepatic veins cups disappeared in the Sm and 1% CLC supplementation groups shown with H&E staining.

Conclusions: Therefore, CLC probably could develop hepatoprotective products against chemical-induced liver damage.

Keywords: Acetaminophen, catalase, Chlorella sp. crude lysate, glutamic oxaloacetic transaminase,
glutamic pyruvic transaminase, hepatoprotective function, superoxide dismutase


Acetaminophen; catalase; Chlorella sp. crude lysate; glutamic oxaloacetic transaminase; glutamic pyruvic transaminase; hepatoprotective function; superoxide dismutase

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Bhola V, Swalaha FM, Nasr M, Bux F. Fuzzy intelligence for

investigating the correlation between growth performance and

metabolic yields of a Chlorella sp. exposed to various flue gas

schemes. Bioresour Technol 2017;243:1078‑86.

Bagul SY, K Bharti R, Dhar DW. Assessing biodiesel quality

parameters for wastewater grown Chlorella sp. Water Sci Technol


Tiron O, Bumbac C, Manea E, Stefanescu M, Nita Lazar M.

Overcoming microalgae harvesting barrier by activated algae

granules. Sci Rep 2017;7:4646.

Zakaria SM, Kamal SMM, Harun MR, Omar R, Siajam SI.

Subcritical water technology for extraction of phenolic

compounds from chlorella sp. microalgae and assessment on its

antioxidant activity. Molecules 2017;22:1105.

Sivaramakrishnan R, Incharoensakdi A. Enhancement of total

lipid yield by nitrogen, carbon, and iron supplementation in

isolated microalgae. J Phycol 2017;53:855‑68.

Kim DY, Lee K, Lee J, Lee YH, Han JI, Park JY, et al.

Acidified‑flocculation process for harvesting of microalgae:

Coagulant reutilization and metal‑free‑microalgae recovery.

Bioresour Technol 2017;239:190‑6.

Ramadass K, Megharaj M, Venkateswarlu K, Naidu R. Toxicity

of diesel water accommodated fraction toward microalgae,

Pseudokirchneriella subcapitata and Chlorella sp. MM3.

Ecotoxicol Environ Saf 2017;142:538‑43.

Nath A, Tiwari PK, Rai AK, Sundaram S. Microalgal consortia

differentially modulate progressive adsorption of hexavalent

chromium. Physiol Mol Biol Plants 2017;23:269‑80.

González‑Sánchez A, Postern C. Fate of H2S during the

cultivation of Chlorella sp. deployed for biogas upgrading.

J Environ Manage 2017;191:252‑7.

Zenooz AM, Ashtiani FZ, Ranjbar R, Nikbakht F, Bolouri O.

Comparison of different artificial neural network architectures in

modeling of Chlorella sp. flocculation. Prep Biochem Biotechnol


Luangpipat T, Chisti Y. Biomass and oil production by Chlorella

vulgaris and four other microalgae ‑ Effects of salinity and other

factors. J Biotechnol 2017;257:47‑57.

Kothari R, Pathak VV, Pandey A, Ahmad S, Srivastava C,

Tyagi VV. A novel method to harvest Chlorellasp. via low

cost bioflocculant: Influence of temperature with kinetic and

thermodynamic functions. Bioresour Technol 2017;225:84‑9

Abdul HZ, Budin SB, Wen Jie N, Hamid A, Husain K,

Mohamed J. Nephroprotective effects of zerumbet Smith ethyl

acetate extract against paracetamol‑induced nephrotoxicity and

oxidative stress in rats. J Zhejiang Univ Sci B 2012;13:176‑85.

Ahmed MB, Khater MR. Evaluation of the protective potential

of Ambrosia maritime extract on acetaminophen‑induced liver

damage. J Ethnopharmacol 2001;75:169‑74.

Zhang Y, Lou JX, Hu XY, Yang F, Hong S, Lin W. Improved

prescription of taohechengqi‑tang alleviates D‑galactosamine

acute liver failure in rats. World J Gastroenterol 2016;22:2558‑65.

Hwang HJ, Kim IH, Nam TJ. Effect of a glycoprotein from

Hizikiafusiformis on acetaminophen‑induced liver injury. Food

Chem Toxicol 2008;46:3475‑81.

Bajt ML, Farhood A, Lemasters JJ, Jaeschke H. Mitochondrial

bax translocation accelerates DNA fragmentation and cell

necrosis in a murine model of acetaminophen hepatotoxicity. J

Pharmacol Exp Ther 2008;324:8‑14.

Jaeschke H, Knight TR, Bajt ML. The role of oxidantstress

and reactive nitrogen species in acetaminophen hepatotoxicity.

Toxicol Lett 2003;144:279‑88.

Jaeschke H, McGill MR, Ramachandran A. Oxidantstress,

mitochondria, and cell death mechanisms in drug‑induced liver

injury: Lessons learned from acetaminophen hepatotoxicity. Drug

Metab Rev2012;44:88‑106.

Kao CY, Chen TY, Chang YB, Chiu TW, Lin HY, Chen CD,

et al. Utilization of carbondioxide in industrial flue gases for

the cultivation of microalga Chlorella sp. Bioresour Technol


Larson AM, Polson J, Fontana RJ, Davern TJ, Lalani E,

Hynan LS, et al. Acetaminophen‑inducedacute liver failure:

Results of a United Statesmulticenter, prospective study.

Hepatology 2005;42:1364‑72.

McGill MR, Sharpe MR, Williams CD, Taha M,

Curry SC, Jaeschke H. The mechanism underlying

acetaminophen‑inducedhe patotoxicity in humans and mice

involves mitochondrial damage and nuclear DNA fragmentation.

J Clin Invest 2012;122:1574‑83.

Yuan HD, Jin GZ, Piao GC. Hepatoprotective effects of

an active part from Artemisia sacrorum Ledeb. againstacet

aminophen‑induced toxicity in mice. J Ethnopharmacol


Fan X, Bai L, Zhu L, Yang L, Zhang X. Marinealgae‑derived

bioactive peptides for humannutrition and health. J Agric Food

Chem 2014;62:9211‑22.

Ramachandran A, Lebofsky M, Weinman SA, Jaeschke H.

The impact of partial manganese superoxide dismutase (SOD2)‑deficiency on mitochondrial oxidant stress, DNA

fragmentation and liverinjury during acetaminophen

hepatotoxicity. Toxicol Appl Pharmacol 2011;251:226‑33.

Ko SC, Kim D, Jeon YJ. Protective effect of a novel

antioxidative peptide purified from a marine Chlorella ellipsoidea

protein against free radical‑induced oxidative stress. Food Chem

Toxicol 2012;50:2294‑302.

Li L, Li W, Kim YH, Lee YW. Chlorella vulgaris extracta

melioratescarbon tetrachloride‑inducedacutehepaticinjury in

mice. Exp Toxicol Pathol 2013;65:73‑80.

Mladenović D, Radosavljević T, Ninković M, Vucević D,

Jesić‑Vukićević R, Todorović V. Liverantioxidantcapacity in the

earlyphase of acuteparacetamol‑inducedliverinjury in mice. Food

Chem Toxicol 2009;47:866‑70.

Morris HJ, Almarales A, Carrillo O, Bermúdez RC. Utilisation of

chlorella vulgaris cell biomass for the production of enzymatic

protein hydrolysates. Bioresour Technol 2008;99:7723‑9.

Pang HY, Chu YC, Chen SJ, Chou ST. Hepatoprotection of

chlorella against carbon tetrachloride‑induced oxidative damage

in rats. In Vivo 2009;23:747‑54.