The Effect of Hydro‑alcoholic Extract of Rheum Turkestanicum Roots against Oxidative Stress in Endothelial Cells

Azar Hosseini, Sahar Sheikh, Mohammad Soukhtanloo, Bizhan Malaekeh- Nikouei, Arezoo Rajabian

Abstract


Introduction: Cardiovascular disorders (CVD) are a common cause of mortality worldwide. Oxidative
stress is thought to be a major factor leading to CVD. Anti‑oxidants such as medicinal plants may
have a role in the mitigation of vascular problems through free radicals scavenging. In this study, we
evaluated the protective effects of Rheum turkestanicum against hydrogen peroxide (H2O2)‑induced
toxicity in endothelial cells (BAE‑1). Methods: To evaluate the protective effect of R. turkestanicum
against H2O2 toxicity, four groups comprised of control group (the cells without any treatment),
H2O2 group (the cells incubated with H2O2 (200 μM)), and treatment groups (the cells treated with
R. turkestanicum (12200 μg/ml) alone or 24h before exposure to H2O2). Quercetin (30.23 μg/ml)
was used as a bioactive ingredient of the extract. Then the cell viability, reactive oxygen species,
lipid peroxidation, and apoptosis were evaluated. Results: H 2O2 exposure reduced cell viability to
13.6 ± 1.6%, enhanced ROS generation to 1445 ± 80.7%, lipid peroxidation (LPO, 290 ± 13% of
control), and apoptotic cells (P < 0.001). In contrast, compared with H2O2 group, R. turkestanicum
and quercetin significantly restored the cell viability to 80.3 ± 1.6 and 87.2 ± 2.1%, ROS
formation to 186 ± 10 and 129 ± 1%, as well as LPO to 130.7 ± 7.7 and 116 ± 2.5 of control,
respectively (P < 0.001). Therefore, the extract reduced H2O2‑induced toxicity in BAE‑1 cells by
scavenging of free radicals. Conclusion: Our findings demonstrated that the extract might reduce
toxicity of endothelial cells by attenuation of oxidative stress, which can be related to the presence
of active ingredients including quercetin.


Keywords


Apoptosis,; endothelial cells; oxidative stress; quercetin; Rheum turkestanicum

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