Effect of Resistance Training on Plasma Nitric Oxide and Asymmetric Dimethylarginine Concentrations in Type I Diabetic Rats

Abstract

Background: Asymmetric dimethylarginine (ADMA) has a predominant role in progression of some cardiovascular diseases, including diabetes. It interferes with L‑arginine in production of nitric oxide (NO) by inhibition of NO synthase. The purpose of this study was to evaluate the effect of resistance training on plasma NO and ADMA concentrations in type 1 diabetic male rats.

Methods: Thirty‑six male wistar rats were randomly divided into four groups: (1) control; (2) diabetic; (3) diabetic trained, and (4) control trained (n = 9 each). In the trained groups, the animals undertook one training session per day, 3 days/week, for 4 weeks. At the end of experiment, blood samples were taken and the concentrations of plasma glucose, insulin, lipid profile, NO and ADMA concentrations were determined.

Results: plasma ADMA concentration showed a significant increase in diabetic rats compare to control group (0.73 ± 0.07 vs. 0.62 ± 0.04 μmol/l; P < 0.05). The plasma ADMA level in the trained diabetic and control were lower than the sedentary groups, although it was not statistically significant. Plasma NO concentration in diabetic group was lower than control (P < 0.05). Resistance training significantly increased plasma NO concentration in diabetic animals (P < 0.05).

Conclusion: Elevated ADMA level in diabetic animals can normalize during resistance exercise. Reduced ADMA level and increased NO level following resistance training might improve cardiovascular risk in diabetic subjects.

Keywords: Asymmetric dimethylarginine, diabetes, nitric oxide, resistance training