Acacetin Attenuates Renal Damage‑Induced by Ischemia‑Reperfusion with Declining Apoptosis and Oxidative Stress in Mice

Abstract

Background: Renal ischemia‑reperfusion disturbs both the function and the histology of this
organ. Acacetin (Aca) is a natural flavonoid that is effective for relief of many diseases. The
aim of this study was to determine the impacts of Aca on renal ischemia‑reperfusion process in
mice.

Methods: In total, 84 male Balb/cmice divided into 12 groups and were administrated
intraperitoneally for 4 days with or without surgery to dimethyl sulfoxide 0.01% or Aca (10, 25,
and 50 mg/kg) as Control, control Acas, sham, sham Acas groups. Ischemia‑reperfusion without
or with Aca (10, 25, and 50 mg/kg) treatments were the other groups. Parameters related to the
function and the histology of the kidneys were evaluated and statistically analyzed from kidney
and blood serum samples in the respect of the groups.

Results: In ischemia‑reperfusion and
ischemia‑reperfusion + Aca (10 mg/kg) groups, there were significantly increased in urea, creatinine,
malondialdehyde (MDA), and apoptosis rate, whereas total antioxidant capacity decreased compared
to the control and sham and ischemia‑reperfusion + Aca (25 and 50 mg/kg) (P < 0.05). The
histopathology alteration was seen in the ischemia‑reperfusion group than the others (P < 0.01).
Moreover, there was a significant difference between ischemia‑reperfusion + Aca (25 and50 mg/kg)
groups than ischemia‑reperfusion + Aca (10 mg/kg) one (P < 0.05).

Conclusions: The recovery
effect of Aca was offered on renal ischemia‑reperfusion damage in a dose‑dependent manner in mice,
showing by kidney histopathology and functional criteria improvements. The attributed mechanism
for this impression would be the antioxidant property of Aca, decreasing both MDA levels and
apoptosis rate in kidney tissue.

Keywords: Acacetin, antioxidants, apoptosis, malondialdehyde, reperfusion injury