Preventive Effect of Ferula asafoetida Oleo Gum Resin on Histopathology in Cuprizone‑Induced Demyelination Mice

Seyyed Majid Bagher, Mohammad Javad Maghsoudi, Maryam Yadegari

Abstract


Background: Ferula asafoetida is introduced as a valuable remedy for hysteria and some other nervous disorders in Iranian traditional medicine. Asafoetida is an oleo‑gum‑resin obtained from the exudates of the roots of the Ferula asafoetida. Previous studies have shown that this oleo gum resin has antioxidant, anti‑apoptosis, and differentiation properties in the nervous system. The aim of this study was to evaluate the effect of asafoetida on the death of oligodendrocytes and demyelination in male C57BL/6 mice in cuprizone (CPZ)‑induced animal model of multiple sclerosis. Methods: Demyelination was induced by oral administration of rats with the 0.2% CPZ that was added to the usual diet for 8 weeks. Animals intraperitoneally received daily asafoetida at doses of 25 or 50 mg/kg of bodyweight simultaneously.   At the end of the weeks, animal brains were removed and fixed to histological studies using Luxol fast blue staining. Asafoetida was screened for its antioxidant activity using 2, 2‑diphenyl‑1‑picrylhydrazyl free radical scavenging assay and for its inhibitory activity against lipid peroxidation catalyzed by soybean lipoxygenase. Results: The results of this study showed that asafoetida significantly decreased infiltration rate in both groups of asafoetida 25 and 50 mg/kg, respectively (P < 0.01). Histological evaluations showed the lower demyelination in LFB in the group treated with asafoetida. Conclusions: The results of this study showed that asafoetida plays a neuro protective role in CPZ models of multiple sclerosis by reducing neuronal demyelination and oligodendrocytes death.

Keywords


Antioxidant; asafoetida; cuprizone; multiple sclerosis

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References


Yoshikawa K, Palumbo S, Toscano C, Bosetti F. Inhibition

of 5‑lipoxygenase activity in mice during CPZ‑induced

demyelination attenuates neuroinflammation, motor dysfunction

and axonal damage. Prostaglandins Leukot Essent Fatty

Acids2011;85:43‑52.

Barnett MH, Prineas JW. Relapsing and remitting multiple

sclerosis: Pathology of the newly forming lesion. Ann Neurol

;55:458‑68.

Abakumova T, Kuz’kina A, Zharova M, Pozdeeva D, Gubskii I,

Shepeleva I, et al. Cuprizone model as a tool for preclinical

studies of the efficacy of multiple sclerosis diagnosis and

therapy. Bull Exp Biol Med 2015;159:111‑5.

Franco‑Pons N, Torrente M, Colomina MT, Vilella E. Behavioral

deficits in the cuprizone‑induced murine model of demyelination/

remyelination. Toxicol Lett 2007;169:205‑13.

Hiremath M, Saito Y, Knapp G, Ting JY, Suzuki K,

Matsushima G. Microglial/macrophage accumulation

during cuprizone‑induced demyelination in C57BL/6 mice.

J Neuroimmunol 1998;92:38‑49.

Bagheri SM, Dashti‑R MH. Influence of asafoetida on prevention

and treatment of memory impairment induced by d‑galactose

and NaNO2 in mice. AmJ Alzheimer’s DisOther Demen

;30:607‑12.

Bagheri SM, Sahebkar A, Gohari AR, Saeidnia S, Malmir M,

Iranshahi M. Evaluation of cytotoxicity and anticonvulsant

activity of some Iranian medicinal Ferula species. Pharm Biol

;48:242‑6.

Iranshahy M, Iranshahi M. Traditional uses, phytochemistry

and pharmacology of asafoetida (Ferula assa‑foetida

oleo‑gum‑resin)—A review. J Ethnopharmacol 2011;134:1‑10.

Bagheri SM, Abdian‑Asl A, Moghadam MT, Yadegari M,

Mirjalili A, Zare‑Mohazabieh F, et al. Antitumor effect of

Ferula assa foetida oleo gum resin against breast cancer

induced by 4T1 cells in BALB/c mice. J Ayurveda Integr Med

;8:152‑8.

Bandyopadhyay D, Basak B, Chatterjee A, Lai TK, Banerji A,

Banerji J, et al. Saradaferin, a new sesquiterpenoid coumarin

from Ferula assafoetida. Nat Prod Res2006;20:961‑5.

Kiasalari Z, Khalili M, Roghani M, Heidari H, Azizi Y.

Antiepileptic and antioxidant effect of hydroalcoholic extract of

ferula assa foetida gum on pentylentetrazole‑induced kindling in

male mice. Basic Clin Neurosci 2013;4:299‑306.

Dehpour AA, Ebrahimzadeh MA, Seyed Fazel N, Seyed

Mohammad N. Antioxidant activity of the methanol extract of

Ferula assafoetida and its essential oil composition. Grasas

Aceites 2009;60:405‑12.

Bafghi AF, Bagheri SM, Hejazian SH. Antileishmanial activity

of Ferula assa‑foetida oleo gum resin against Leishmania major:

An in vitro study. J Ayurveda Integr Med 2014;5:223‑6.

Saleem M, Alam A, Sultana S. Asafoetida inhibits early

events of carcinogenesis: Achemopreventive study. Life Sci

;68:1913‑21.

Bagheri SM, Rezvani ME, Vahidi AR, Esmaili M. Anticonvulsant

effect of ferula assa‑foetida oleo gum resin on chemical and

amygdala‑kindled rats. N Am J Med Sci 2014;6:408‑12.

Abu‑Zaiton AS. Anti‑diabetic activity of Ferula assafoetida

extract in normal and alloxan‑induced diabetic rats. Pak J Biol

Sci 2010;13:97‑100.

Bagheri S, Hejazian S, Dashti‑R M. The relaxant effect of seed

fs essential oil and oleo‑gum‑resin of ferula assa‑foetida on

isolatedrat’s ileum. Ann Med Health Sci Res2015;4:238‑41.

Fatehi M, Farifteh F, Fatehi‑Hassanabad Z. Antispasmodic

and hypotensive effects of Ferula asafoetida gum extract.

J Ethnopharmacol 2004;91:321‑4.

Bagheri S, Dashti‑R M, Morshedi A. Antinociceptive effect

of Ferula assa‑foetida oleo‑gum‑resin in mice. Res Pharm Sci

;9:207‑12.

Azizian H, Ebrahim Rezvani M, Esmaeilidehaj M, Bagheri SM.

Anti‑obesity, fat lowering and liver steatosis protective effects

of ferula asafoetida gum in type 2 diabetic rats: Possible

involvement of leptin. IJDO 2012;4:120‑6.

Bagheri SM, Yadegari M, Mirjalily A, Rezvani ME. Evaluation

of toxicity effects of asafetida on biochemical, hematological,

and histological parameters in male wistar rats. Int J Toxicol

;22:61‑5.

Haghmorad D, Mahmoudi MB, Mahmoudi M, Rab SZ, Rastin M,

Shegarfi H, et al. Calcium intervention ameliorates experimental

model of multiple sclerosis. Oman Med J 2014;29:185‑9.

Chitnis T, Imitola J, Wang Y, Elyaman W, Chawla P, Sharuk M,

et al. Elevated neuronal expression of CD200 protects Wlds

mice from inflammation‑mediated neurodegeneration. Am J

Pathol 2007;170:1695‑712.

Bagheri SM, Hedesh ST, Mirjalili A, Dashti‑R MH. Evaluation

of anti‑inflammatory and some possible mechanisms of

antinociceptive effect of ferula assa foetida oleo gum resin.

J Evid Based Complementary Altern Med 2015;21:271‑6.

Manto M. Abnormal copper homeostasis: Mechanisms and roles

in neurodegeneration. Toxics 2014;2:327‑45.

Omotoso GO, Gbadamosi IT, Afolabi TT, Abdulwahab AB,

Akinlolu AA. Ameliorative effects of Moringa on

cuprizone‑induced memory decline in rat model of multiple

sclerosis. Anat Cell Biol 2018;51:119‑27.

Acs P, Kipp M, Norkute A, Johann S, Clarner T, Braun A,

et al. 17β-estradiol and progesterone prevent cuprizone

provoked demyelination of corpus callosum in male mice. Glia

;57:807‑14.

Tayeboon GS, Tavakoli F, Hassani S, Khanavi M, Sabzevari O, Ostad SN. Effects of cymbopogon citratus and Ferula

assa‑foetida extracts on glutamate‑induced neurotoxicity. In Vitro

Cell Dev Biol Anim 2013;49:706‑15.

Moghadam FH, Zarch BV, Shafiei M. Double edged effect of

gum‑resin of ferula assa‑foetida on lifespan of neurons. Iran J

Basic Med Sci 2013;16:668‑71.

Moghadam FH, Dehghan M, Zarepur E, Dehlavi R,

Ghaseminia F, Ehsani S, et al. Oleo gum resin of Ferula

assa‑foetida L. ameliorates peripheral neuropathy in mice.

J Ethnopharmacol2014;154:183‑9.

Lee SC, Tsai CC, Yao CH, Chen YS, Wu MC. Ferulic acid

enhances peripheral nerve regeneration across long gaps. Evid

Based Complement Alternat Med 2013;2013:876327.

Palumbo S, Toscano C, Parente L, Weigert R, Bosetti F.

Time‑dependent changes in the brain arachidonic acid cascade

during cuprizone‑induced demyelination and remyelination.

Prostaglandins Leukot Essent Fatty Acids 2011;85:29‑35.

Kelsey NA, Wilkins HM, Linseman DA. Nutraceutical antioxidants

as novel neuroprotective agents. Molecules 2010;15:7792‑814.

Chen C, Kong A‑NT. Dietary chemopreventive compounds and

ARE/EpRE signaling. Free Radic Res 2004;36:1505‑16.

Cheng CY, Su SY, Tang NY, Ho TY, Chiang SY, Hsieh CL. Ferulic

acid provides neuroprotection against oxidative stress‑related

apoptosis after cerebral ischemia/reperfusion injury by inhibiting

ICAM‑1 mRNA expression in rats. Brain Res 2008;1209:136‑50.

Motai T, Kitanaka S. Sesquiterpene phenylpropanoids from

Ferula fukanensis and their nitric oxide production inhibitory

effects. J Nat Prod 2005;68:365‑8.