The Effects of Calorie Restriction and Time‑Restricted Feeding on IGF1 Serum Level and Lipid Profile in Male Wister Rats with Previous Obesity

Nazanin Asghari Hanjani, Negar Zamaninour, Narjes Najibi, Agha Fatemeh Hosseini, Farinaz Nasirinezhad, Mohammad Reza Vafa


Background: Calorie restriction (CR) is known as a nutritional gold standard for life extension and different studies have shown that insulin‑like growth factor (IGF1) reduction through CR may be involved in CR’s anti‑aging effects. Besides, time‑restricted‑feeding (TRF) is also highlighted due to more feasibility and positive health effects. We designed this study to compare the effects of CR and TRF on IGF1 and other metabolic parameters. Methods: Fifty‑two male Wistar rats (3 weeks old) were subjected to either a control (CON, n = 11) diet or high‑fat diet (HFD, n = 42) for 17 weeks. In the second phase of the study, the HFD group were divided into four groups (n = 9) 1) 30% CR, 2) Night Intermittent Fasting (NIF, active phase), 3) day intermittent fasting (DIF, rest phase), and 4) Ad‑Libitum (AL) with a standard diet for 10 weeks. Blood samples were collected at the end of both phases. Results: HFD increased IGF1 and deteriorated lipid profiles, except for triglycerides (P: 0.018,, 0.032) but CR in these obese subjects could not lower the IGF1 level. HDL significantly decreased in DIF compared to CON and CR (P; 0.001). Meanwhile, HOMA‑IR increased in DIF and was significant compared to CR (P: 0.002). Serum glucose levels decreased in CR compared to all groups except for CON (P: 0.001). Conclusion: Data indicates the role of previous obesity on the effect of CR on the IGF1 level and highlights the effect of inappropriate time of food intake on HDL and APOA1.


Apo A‑I; calorie restriction; IGF‑1; intermittent fasting

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