Antifungal Effects of Iranian Propolis Extract and Royal jelly Against Candida albicans In‑Vitro

Hassan Moghim, Simin Taghipour, Soleiman Kheiri, Hamideh Khabbazi, Azar Baradaran

Abstract


Background: Candida albicans is the most important opportunistic fungal that can establish infection in susceptible individuals. Iranian Propolis and Royal jelly are bee products that are traditionally used against fungal infections. This study was aimed to evaluate the antifungal effects of Iranian Propolis extract and Royal jelly against C. albicans in vitro. Methods: Antifungal activities of the extracts were performed according to microbroth dilution method in 96‑well microdilution plates. The amount of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) based on counting the number of fungal colonies (CFU) were evaluated for each of Royal jelly and Iranian Propolis extracts against C. albicans compared with the control group. Results: In this study, the MIC, MIC50, and MFC of Royal jelly on C. albicans were, respectively, 80, 103 ± 25, and 160 ± 34 mg/mL and for the Iranian Propolis alcoholic extract were, respectively, 0.030 ± 0.015, 0.0618 ± 0.027, and 0.0833 ± 0.0599 mg/mL. Conclusions: The results indicate that both Royal jelly and Iranian Propolis alcoholic extract are effective against C. albicans, but the former species has higher antifungal activity. If the clinical trials confirm the results of this study, Iranian propolis, as a new antifungal agent by replacing chemical drugs, can be used to develop antifungal medicinal herbs.

Keywords


Candida albicans; Iranian propolis; Royal jelly

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References


Rippon JW. Candidiasis and the Pathogenic Yeasts. Medical

Mycology. 3rd ed. Philadelphia: W.B. Saunders Company; 1988.

p. 532‑8.

Moghim H, Taghipour S, Shahinfard N, Kheiri S, Khabbazi H.

Comparative study on the antifungal activity of hydroalcoholic

extract of Iranian Propolis and Royal jelly against Rhizopus

oryzae. J Herbmed Pharmacol 2015;4:89‑92.

Moghim H, Taghipour S, Shahinfard N, Kheiri S, Panahi R.

Antifungal effects of Zataria multiflora and Nigellasativa

extracts against Candida albicans. J Herbmed Pharmacol

;4:138‑141.

Yuan H, Ma Q, Ye L, Piao G. The traditional medicine and

modern medicine from natural products. Molecules 2016;21:559.

Fratini F, Cilia G, Mancini S, Felicioli A. Royal Jelly: An ancient

remedy with remarkable antibacterial properties. Microbiol Res

;192:130‑41.

Danciu C, Avram S, Pavel IZ, Ghiulai R, Dehelean CA, Ersilia A,

et al. Main isoflavones found in dietary sources as natural

anti‑inflammatory agents. Curr Drug Targets 2018;19:841‑53.

Rafieian‑Kopaie M, Baradaran A. Plants antioxidants: From

laboratory to clinic. J Nephropathol 2013;2:152‑3.

Pasupuleti VR, Sammugam L, Ramesh N, Gan SH. Honey,

Propolis, and Royal Jelly: A Comprehensive Review of Their

Biological Actions and Health Benefits. Oxid Med Cell Longev.

;2017:1259510. doi: 10.1155/2017/1259510. Epub 2017 Jul

PMID: 28814983; PMCID: PMC5549483.

Nasri H. Herbal drugs and new concepts on its use. J Prev

Epidemiol 2016;1:e01.

Badiee P, Hashemizadeh Z. Opportunistic invasive fungal

infections: Diagnosis & clinical management. Indian J Med Res

;139:195‑204.

Singhi SC, Reddy TC, Chakrabarti A. Candidemia in a pediatric

intensive care unit. Pediatr Crit Care Med 2004;5:369‑74.

Pfaller MA, Diekema DJ. Rare and emerging opportunistic fungal

pathogens: Concern for resistance beyond Candida albicans and

Aspergillus fumigatus. J Clin Microbiol 2004;42:4419‑31.

Bruce Diemond J, Lopez C, Huerta Romano F, Montiel

Castillo C. Infeccion por Candida en el paciente pediatrico

inmunocomprometido [Fungal (Candida) infections in the

immunocompromised pediatric patient]. Drugs Today (Barc)

;44(Suppl 4):45‑51.

Sayyadi F, Mahdavi S, Moghadamnia AA, Moslemi D, Shirzad A,

Motallebnejad M. The effect of aqueous and ethanolic extract of

Iranian propolis on Candida Albicans isolated from the mouth of

patients with colorectal malignancy undergone chemotherapy:

An in‑vitro study. Caspian J Intern Med 2020;11:62‑6.

Bankova V, Popova M, Trusheva B. Propolis volatile compounds:

chemical diversity and biological activity: A review. Chem Cent

J 2014;8:28.

Hajian S. Positive effect of antioxidants on immune system.

Immunopathol Persa 2015;1:e02.

Al‑Huqail AA, Behiry SI, Salem MZM, Ali HM, Siddiqui MH,

Salem AZM. Antifungal, antibacterial, and antioxidant activities of

acacia saligna (Labill.) H. L. Wendl. Flower extract: HPLC analysis

of phenolic and flavonoid compounds. Molecules 2019;24:700.

Tolouian A, Khosravian M, Ragati Haghi H, Bolourian A,

Mojtahedi Z, Asgharpour M. Herbal medicines in the treatment

of coronavirus disease 2019 (COVID-19). J Nephropharmacol.

;10:e18.

Rafieian‑Kopaei M, Asgharzadeh S, Tamadon MR, Yalameha B.

Ameliorative effect of royal jelly against nephrotoxicity induced

by gentamicin. Ann Res Antioxid 2018;3:e05.

Jamali F, BaserisalehiMand B. Antimicrobial activity and

chemical screening of propolis extracts. Am J Life Sci

;2:72‑5.

Asgari A. Herbal medicines and kidney; friends or foes? J

Nephropharmacol 2014;3:5‑6.