Protective Effect of Vitamin D Supplementation on Some Hemogram Derived Inflammatory Indices in Normal and High‑Fat Diet Fed Male Wistar Rats

Fahimeh Agh, Seyed H. Mousavi, Naheed Aryaeian, Fatemehsadat Amiri, Mohammad R. Jalilvand, Leila Janani, Motahareh Hasani, Fatemeh Sepahvand, Fahimeh Zamani‑Garmsiri


Background: Hematological inflammatory indices are currently suggested to assess systemic inflammation. This study aims to investigate a vitamin D supplementation effect on hematological indices of inflammation in rats. Method: Forty‑eight middle‑aged male rats were allocated into a normal diet (ND) group (10% fat) and a high‑fat diet (HFD) group (60% fat). The animals were fed for 26 weeks. After this period, each group was randomly divided into three subgroups, each of 8 rats: Group (1): animals were fed the ND and HFD containing 1 IU/g vitamin D for 4 months, group (2): animals were fed the ND and HFD containing 6 IU/g vitamin D for 4 months and group (3): animals were euthanized to evaluate the HFD effect. Serum 25‑hydroxyvitamin D level, white blood cell count (WBCs), platelet count, platelet crit (PCT), mean platelet volume (MPV), platelet distribution width (PDW), platelet‑to‑lymphocyte ratio (PLR), neutrophil‑to‑lymphocyte ratio (NLR), monocyte‑to‑lymphocyte ratio (MLR) were measured. Results: The HFD, significantly increased body weight, PCT, PDW, PLR, NLR, and MLR and significantly reduced serum vitamin D levels compared to the ND (P < 0.05). There was a significant decrease in food intake, MPV, PDW, and NLR after vitamin D supplementation in the ND‑fed group (P < 0.05). A significant reduction in platelet count, PCT, and MLR was observed after vitamin D supplementation in HFD‑fed rats (P < 0.05). Conclusions: In our study, some hemogram‑derived inflammatory indices were higher in the HFD‑fed group, and vitamin D supplementation lowering effects on some hematological indices were seen in both ND and HFD groups.


Blood cell count; high‑fat diet; inflammation; vitamin D

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